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You are here:Home » Growth Factors, Cytokines » Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Equine (TNFa)

Tumor Necrosis Factor alpha, Recombinant, Equine (TNFa)

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Specifications

Catalog #T9160-02D
SourceRecombinant protein expressed in Pichia pastoris
DescriptionEquine Tumour necrosis factor alpha (TNF alpha) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the equine inflammatory response.
Biological ActivityThis product has an ED50 < 0.05ng/ml, determined by measuring the cytolytic effect of recombinant equine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D.
Endotoxin Level:
0.15EU/ug
ApplicationsSuitable for use in In vitro applications and ELISA. Other applications not tested.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume and store at -20°C. Reconstituted product is stable for 12 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17.1kD
Purity>95% by SDS PAGE analysis.
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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