| Tumor necrosis factor alpha (TNF-a), also called cachectin, consists of 158 amino acids. TNF-a is a 17.5kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-a is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Additional amino acid, methionine, was attached at N-terminus of the protein. |
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| Catalog # | T9160-03H |
| Amino Acid Sequence | MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY |
| SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY |
| LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL |
| Biological Activity | Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is <0.16ng/ml. |
| Activity Assay | L929 cytotoxicity assay using actinomycin D |
| Cell Number: 2 x 10,000 cells/well |
| Incubation: 24hr after sample treatment |
| Assay Media: 5% FBS media |
| Cytokine Concentration: 12 pg/ml - 25 ng/ml |
| Detection method: MTT assay |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Molecular Weight | 17.5kD |
| Source | Human, Recombinant, E. coli |
| Purity | > 95% by SDS PAGE. Purified by using conventional chromatography techniques. Endotoxin: 1EU/ug protein (LAL method) |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4 |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
