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You are here:Home » Molecular Biology » Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)

Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)

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Specifications

Tumor Necrosis Factor-alpha (TNFa) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF-alpha is a 17.4kD protein containing 157 amino acid residues.
Catalog #T9160-10
Biological ActivityHuman TNF-alpha is fully biologically active when compared to standards. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is 0.05ng/ml, corresponding to a specific activity of 2x10e7 units/mg.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17.4kD
SourceHuman, recombinant expressed in E. coli
Purity98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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