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You are here:Home » Molecular Biology » Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)

Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)

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Specifications

Recombinant Human Tumor Necrosis Factor alpha is a single, non-glycosylated, polypeptide chain containing 158aa and having a molecular weight of 17.5kD. The sequence of the first five N-terminal amino acids was determined to be Met-Val-Arg-Ser-Ser.
Catalog #T9160-10D
Biological ActivityThe ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is a specific activity of 5x10e7 IU/mg.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with ddH2O, 0.1% HSA or BSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17.5kD
SourceE. coli
Purity95% by RP-HPLC and SDS-PAGE
FormSupplied as a lyophilized powder from 20mM PBS, pH 7.2, 100mM sodium chloride. Reconstitute with sterile ddH2O to a concentration 100ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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