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Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)
Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)
Pricing
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| Human TNF is a 17kD factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26kD form of TNF is expressed as a membrane bound molecule. TNF is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF have been observed. | | | Catalog # | T9160-10E | | Source | Escherichia coli. | | Biological Activity | The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is 0.05ng/ml, corresponding to a Specific Activity of 2 x10e7 IU/mg. | | Endotoxin | 1EU/ug (LAL) | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute with sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 1 month at 4 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~17.5kD | | Source | E. coli | | Purity | 97% as determined by SDS-PAGE and HPLC. | | Form | Supplied as a lyophilized powder from 15mMNa2HPO4, 0.3M sodium chloride. Reconstitute with sterile dH2O to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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