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You are here:Home » Growth Factors, Cytokines » Growth Factors-TNF » Tumor Necrosis Factor alpha, variant, Recombinant, Human (TNFa-variant)

Tumor Necrosis Factor alpha, variant, Recombinant, Human (TNFa-variant)

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Specifications

The clinical use of the potent antitumor activity of TNF-a has been limited by the proinflammatory side effects including fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-a mutants with low systemic toxicity has been an intense pharmacological interest. Human TNF-a, which binds to the murine TNF-R55 but not to the murine TNF-R75, exhibits retained antitumor activity and reduced systemic toxicity in mice compared with murine TNF-a, which binds to both murine TNF receptors. Based on these results, many TNF-amutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro, and exhibited lower systemic toxicity in vivo.
Catalog #T9160-10G
Biological ActivityFully biologically active when compared to standard. The Specific Activity is 1.0 × 10e8 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Endotoxin 1EU/ug as determined by LAL method.
Storage and StabilityAliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Molecular Weight16 kD
SourceEscherichia coli
Purity~95% by SDS-PAGE and HPLC analyses.
Concentration1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.5. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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