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You are here:Home » Molecular Biology » Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)

Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)

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Specifications

TNF, alpha is an E. coli-derived recombinant protein corresponding to the C-terminal 157aa of human Tumor Necrosis Factor Alpha (TNFa), a cytokine which exerts cytotoxic effects on a wide range of tumor cells.
Catalog #T9160-10H
ED50 0.05 ng/ml as determined by an assay measuring cytolysis of L929 cells in the presence of Actinomycin D.
SourceRecombinant corresponding to human TNF, alpha, expressed in E. coli.
Molecular Weight~17kD (157aa sequence)
Endotoxin 0.1ng/ug
ApplicationsSuitable for use in ELISA, Functional Assays and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.2-0.4ng/well
Western Blot: 1.5-3ng/lane
Functional Assays: 0.05-20ng/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17kD
SourceHuman
PurityPurified: ~98% (SDS PAGE, HPLC).
ConcentrationAs reported
FormSupplied as a lyophilized powder from 3mM tris, pH8.0. No preservative added. Reconstitute with 500ul sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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