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Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)
Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)
Pricing
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| TNF, alpha is an E. coli-derived recombinant protein corresponding to the C-terminal 157aa of human Tumor Necrosis Factor Alpha (TNFa), a cytokine which exerts cytotoxic effects on a wide range of tumor cells. | | | Catalog # | T9160-10H | | ED50 | 0.05 ng/ml as determined by an assay measuring cytolysis of L929 cells in the presence of Actinomycin D. | | Source | Recombinant corresponding to human TNF, alpha, expressed in E. coli. | | Molecular Weight | ~17kD (157aa sequence) | | Endotoxin | 0.1ng/ug | | Applications | Suitable for use in ELISA, Functional Assays and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 0.2-0.4ng/well | | Western Blot: 1.5-3ng/lane | | Functional Assays: 0.05-20ng/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~17kD | | Source | Human | | Purity | Purified: ~98% (SDS PAGE, HPLC). | | Concentration | As reported | | Form | Supplied as a lyophilized powder from 3mM tris, pH8.0. No preservative added. Reconstitute with 500ul sterile dH2O. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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