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You are here:Home » Growth Factors, Cytokines » Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)

Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)

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Specifications

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
Catalog #T9160-15A
Recombinant murine TNF-alpha is a single, glycosylated, polypeptide chain containing 157 amino acids
Amino Acid SequenceMLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Biological ActivityThe ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is <0.1ng/ml, corresponding to a Specific Activity of 10x10e6U/mg.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.24 as the extinction coefficient for a 0.1% (1mg/ml) solution.
2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17301.32D
SourceRecombinant, murine expressed in E.coli.
Purity97% by RP-HPLC and SDS-PAGE.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O, 0.1% BSA or HSA to 0.1mg/ml.
intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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