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You are here:Home » Growth Factors, Cytokines » Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)

Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)

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Specifications

Recombinant murine TNF-alpha produced in S. lividians is a single, non-glycosylated, polypeptide chain containing 156 amino acids and having a molecular mass of 17301.32D.
Catalog #T9160-15C
Biological Activity rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x10e8 IU/mg.
AA SequenceMLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17.4kD
SourceRecombinant, murine, E. coli
Purity98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
FormSupplied as a lyophilized powder in 50mM Tris-HCL pH-8 and 20mM NaCL. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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