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Tumor Necrosis Factor alpha, Recombinant, Porcine (TNFa)
Tumor Necrosis Factor alpha, Recombinant, Porcine (TNFa)
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| Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. | | | Catalog # | T9160-15P | | Source | Recombinant corresponding to porcine TNF-a expressed in E. coli. | | AASequence | LRSSSQTSDK PVAHVVANVK AEGQLQWQSG YANALLANGV KLKDNQLVVP TDGLYLIYSQ | | VLFRGQGCPS TNVFLTHTIS | | RIAVSYQTKV NLLSAIKSPC QRETPEGAEA KPWYEPIYLG GVFQLEKDDR LSAEINLPDY LDFAESGQVY FGIIAL | | Biological Activity | ED50 range=0.001-0.015ng/ml, determined by the dose dependent cytotoxicity of porcine PK15 cells. Optimal concentration for individual application should be determined by a dose response assay. | | Specific Activity | 1.0x10e9-6.6x10e7units/mg | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~17.1kD | | Purity | ~95% (SDS-PAGE). Endotoxin: 0.1ng/ug | | Form | Supplied as a lyophiized powder. Carrier free. | | Reconstitution | Reconstitute with sterile ddH2O to 0.1-1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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