Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Growth Factors, Cytokines » Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Porcine (TNFa)

Tumor Necrosis Factor alpha, Recombinant, Porcine (TNFa)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS . Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2 , GM-CSF.
Catalog #T9160-15P1
DescriptionRecombinant Porcine TNF-a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17,274 Dalton. Porcine TNF-alpha is purified by standard chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityPorcine TNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytotoxicity of porcine PK15 cells was found to be < 0.008ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of Porcine TNF-alpha.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.26 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TNF-alpa as a Reference Standard.
SolubilityIt is recommended to reconstitute the lyophilized Porcine TNF-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Porcine TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution TNF-alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Molecular Weight17.27kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
ConcentrationAs reported
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links