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You are here:Home » Growth Factors, Cytokines » Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Mouse (TNF-alpha, TNFalpha, Tnfa, TNF-a, Cachectin, DIF, MGC151434, Tumor Necrosis Factor, Tnf, Tnfsf1a, Tumor Necrosis Factor Ligand Superfamily Member 2, Tnfsf2)

Tumor Necrosis Factor alpha, Recombinant, Mouse (TNF-alpha, TNFalpha, Tnfa, TNF-a,
Cachectin, DIF, MGC151434, Tumor Necrosis Factor, Tnf, Tnfsf1a, Tumor Necrosis Factor
Ligand Superfamily Member 2, Tnfsf2)

Pricing

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Specifications

Catalog #T9160-15Q
SourceRecombinant corresponding to 156aa of human Tumor Necrosis Factor-alpha expressed in S.Lividians is a single, non-glycosylated, polypeptide chain.
Molecular Weight~17.3kD
Biological ActivityThe ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x108IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a calibrated solution of TNF- as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17.3kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
ConcentrationNot determined
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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