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You are here:Home » Growth Factors, Cytokines » Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Human (TNF-alpha, TNFA, TNF-a, Cachectin, DIF, Tumor Necrosis Factor, TNF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFSF2)

Tumor Necrosis Factor alpha, Recombinant, Human (TNF-alpha, TNFA, TNF-a, Cachectin, DIF,
Tumor Necrosis Factor, TNF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFSF2)

Pricing

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Specifications

Catalog #T9160-15R
SourceRecombinant corresponding to 158aa of human Tumor Necrosis Factor-alpha expressed in E.coli is a single, non-glycosylated, polypeptide chain.
Molecular Weight~17.5kD
Biological ActivityThe ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 2x107IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.24 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TNF-alpha as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17.5kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
ConcentrationNot determined
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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