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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -Tumor Necrosis Factor alpha (TNFa)

Anti -Tumor Necrosis Factor alpha (TNFa)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Catalog #T9160-18A
ApplicationsSuitable for use in Immunohistochemistry, ELISA and Inhibition of biological activity of TNFa in different biological fluids. Other applications not tested.
Recommended DilutionsImmunohistochemistry: Formalin-fixed frozen tissues
Optimal dilutions to be determined by the researcher.
Matched PairCapture: T9160-32A
Detection: T9160-18A
HybridomaX63.Ag8.653 myeloma cells with spleen cells of Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5E852
HostMouse
SourceHuman
Concentration~8mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman recombinant tumor necrosis factor of alpha type.
SpecificityRecognizes human TNFa. Effective in the inhibiting the biological activity of recombinant and naturtal TNFa.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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