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You are here:Home » Molecular Biology » Growth Factors-TNF » Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)

Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)

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Specifications

Tumor Necrosis Factor-alpha (TNF-alpha) is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF superfamily of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation and diabetes.
Catalog #T9160-20
Recombinant rat TNF-alpha is a soluble 17.3kD protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein containing 157 amino acid residues.
Biological ActivityThe ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is 0.05ng/ml, corresponding to a specific activity of
5x10e7 units/mg.
AA SequenceMLRSSSQNSS DKPVVHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
Storage and Stability Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Molecular Weight17.3kD
SourceE. coli
Purity98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
FormSupplied as a lyophilized powder from 0.5X PBS, pH 7.1 (0.2um filtered). Reconstitute in sterile dH2O, carrier protein to 0.1-1mg/ml. Do not vortex.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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