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Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)
Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)
Pricing
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| Recombinant Rat TNF-alpha is a single, non-glycosylated, polypeptide chain containing 157aa and having a molecular weight of 17.33kD. The sequence of the first five N-terminal aa was determined to be Met-Leu-Arg-Ser-Ser. Contains less than 1% dimers and aggregates. | | | Catalog # | T9160-20D | | Source | | | E. coli | | Molecular Weight | ~17.33kD | | Biological Activity | The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is <0.05ng/ml, corresponding to a specific activity of 5x10e7 IU/mg. | | Endotoxin | 0.1ng/ug | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O, 0.1% HSA or BSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 17.33kD | | Source | E. coli | | Purity | 97% pure (HPLC, FPLC and reducing and non-reducing SDS-PAGE). | | Form | Supplied as a lyophilized powder. Reconstitute using sterile deionized water to a concentration 100ug/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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