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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -Tumor Necrosis Factor alpha (TNFa)

Anti -Tumor Necrosis Factor alpha (TNFa)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
TNF-a is a macrophage/monocyte cytokine, usually found as a soluble secreted protein, which has diverse immunomodulatory, anti-tumor and toxic effects. Neutralizes the recombinant TNF-a mediated cytotoxicity of L929 cells and inhibits the tumor growth in mice. Protects mice against the toxicity of recombinant human TNF-a.
Catalog #T9160-32A
ApplicationsSuitable for use in Immunohistochemistry, ELISA and Inhibition of biological activity of TNFa in different biological fluids. Other applications not tested.
Recommended DilutionsImmunohistochemistry: Formalin-fixed frozen tissues
Optimal dilutions to be determined by the researcher.
Matched PairCapture: T9160-32A
Detection: T9160-18A
HybridomaX63.Ag8.653 myeloma cells with spleen cells of Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.152
HostMouse
SourceHuman
Concentration~3mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman recombinant tumor necrosis factor of alpha type.
SpecificityRecognizes human TNFa. Effective in the inhibiting the biological activity of recombinant and naturtal TNFa.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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