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You are here:Home » Molecular Biology » Growth Factors-TNF » Tumor Necrosis Factor beta, Recombinant, Human (TNFb)

Tumor Necrosis Factor beta, Recombinant, Human (TNFb)

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Specifications

Recombinant Human TNF-beta (Lymphotoxin) is a single, non-glycosylated, polypeptide chain containing 171 amino acids and having a molecular mass of 18645 Dalton. Recombinant Human Tumor Necrosis Factor-beta is purified by standard chromatographic techniques.
Catalog #T9160-41
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Arg-Ser-Ser.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityTNF-beta is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 2 x10e6 IU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of TNF-beta.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.082 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TNF-beta as a Reference Standard.
ReconstitutionReconstitute the lyophilized TNF-beta in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. No additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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