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You are here:Home » Molecular Biology » Growth Factors-TNF » Tumor Necrosis Factor beta, Recombinant, Human (TNF-beta, TNFb, Lymphotoxin-alpha, LT-alpha, LTA, LT, Tumor Necrosis Factor Ligand Superfamily Member 1, TNFSF1)

Tumor Necrosis Factor beta, Recombinant, Human (TNF-beta, TNFb, Lymphotoxin-alpha,
LT-alpha, LTA, LT, Tumor Necrosis Factor Ligand Superfamily Member 1, TNFSF1)

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Specifications

Catalog #T9160-41F
SourceRecombinant corresponding to 171aa of human Tumor Necrosis Factor-beta (Lymphotoxin) expressed in E. coli is a non-glycosylated, polypeptide chain.
Molecular Weight~18.6kD
Biological ActivityThe ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 2x10 IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein Content:
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.082 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TNF-beta as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~18.6kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
ConcentrationNot determined
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesLymphotoxin alpha (TNF Superfamily, Member 1)


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