Tumor Necrosis Factor beta, Recombinant, Human (TNF-beta, TNFb, Lymphotoxin-alpha,
LT-alpha, LTA, LT, Tumor Necrosis Factor Ligand Superfamily Member 1, TNFSF1)
Pricing
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| Catalog # | T9160-41F | | Source | Recombinant corresponding to 171aa of human Tumor Necrosis Factor-beta (Lymphotoxin) expressed in E. coli is a non-glycosylated, polypeptide chain. | | Molecular Weight | ~18.6kD | | Biological Activity | The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 2x10 IU/mg. | | Dimmers and Aggregates | 1% (SDS-PAGE) | | | | Protein Content: | | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.082 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TNF-beta as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~18.6kD | | Purity | ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) | | Concentration | Not determined | | Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Lymphotoxin alpha (TNF Superfamily, Member 1) |
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