| TRAF6 is a signalling adaptor protein, which is common to both TLR and TNFR signalling pathways. TRAF6 has been shown to interact with interleukin-1 receptor-associated kinase 1 and 2 (IRAK1 and 2) and also to interact with TNFR family members CD40 and RANK. |
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| Catalog # | T9161-69 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 1-2ug/ml, detects a band of approximately 60kD in PC-3 cell lysates. |
| Optimal dilution determined by the researcher. |
| Recommended Secondary Reagents | I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb |
| I1904-40J: IgG, Fc (HRP) Pab Gt xRb |
| I1904-40P: IgG, Fc, X-Adsorbed (HRP) Pab Gt xRb |
| Recommended Positive Control | PC-3 Cell Total Protein Lysate |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to fourteen amino acid residues within the C-Terminal region of human TRAF6. |
| Specificity | Recognizes human TNF receptor-associated factor 6 (TRAF6). Species Crossreactivity: mouse and rat |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
