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You are here:Home » Molecular Biology » Growth Factors-TNF » Tumor Necrosis Factor Receptor 2 Soluble, Recombinant, Human (sTNFR 2)

Tumor Necrosis Factor Receptor 2 Soluble, Recombinant, Human (sTNFR 2)

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Specifications

TNFRII is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF-alpha but manifests a lower affinity to TNF-beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. Soluble TNFRII is capable of inhibiting TNF-alpha induced activities by acting as a decoy receptor. Recombinant human sTNFRII is an 18.9kD protein (174 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFRII protein.
Catalog #T9162-75
Biological ActivityDetermined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25ng/ml of recombinant human TNF-alpha, is 0.125ug/ml.
Amino Acid SequenceMAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight18.9kD
SourceRecombinant corresponding to 174aa from the cysteine rich ligand binding portion of the extracellular domain of the human TNFRII protein expressed in E. coli
Purity98% by SDS-PAGE and HPLC. Endotoxin:
0.1ng/ug (1EU/ug)
FormSuppllied as a lyophilized powder from 10mM sodium phosphate, pH 8.0, 50mM sodium chloride. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.


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