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You are here:Home » Antibodies » Abs to Tumor Suppressors » Anti -Tuberin, phosphorylated (Ser1254) (TSC2, Tuberous sclerosis 2 protein)

Anti -Tuberin, phosphorylated (Ser1254) (TSC2, Tuberous sclerosis 2 protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Tuberin, a product of the tumor suppressor gene TSC2, is important in regulating cell proliferation and tumor development (1). Mutation in either TSC1or TSC2 is responsible for tuberous sclerosis complex (TSC), an autosomal dominant disorder (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB(3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (35). Additionally, tuberin inhibits the mammalian target of rapamycin (mTOR), resulting in inhibition of p70 S6 kinase and activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation) and, thus, inhibition of translation (3, 67). p38-activated kinase MK2 (MAPKAPK-2) phosphorylates Ser1254 of tuberin, and thus augments the interaction between tuberin and 14-3-3 (8).
Catalog #T9170-06
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5,, 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser1254 of human tuberin.
SpecificityDetects endogenous levels of tuberin only when phosphorylated at serine 1254. Does not detect tuberin phosphorylated at other sites.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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