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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Tyrosine, Phosphorylated

Anti -Tyrosine, Phosphorylated

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Protein phosphorylation and dephosphorylation reactions is a key postranslational event in the modification of protein functions. Protein phosphorylation occurs at tyrosine, serine, or threonine (p-tyr, p-ser, or p-thr) residues. Many different mitogenic systems, such as the EGF, PDGF, and insulin receptor systems contain tyr/ser/thr kinase domains which autophosphorylate specific tyr, ser or thr residues upon binding of their ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated proteins. An understanding of transformation by oncogenes and mitogenic processes of growth factors requires a delineation of all potential partners involved in phosphorylation cascade. The availability of antibodies specific for p-tyr, p-thr or p-thr residues has greatly advanced the studies on role of phosphorylated proteins.
Catalog #T9230-04B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:1000-1:5000
Western Blot: 1:1500-1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C162
HostMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenO-Phospho-L-tyrosine (KLH).
SpecificityRecognizes phosphorylated tyrosine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not react with nonphosphorylated tyrosine, phosphorylated serine or threonine, AMP, or ATP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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