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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Tyrosine, phosphorylated (Phospho-Tyrosine) (HRP)

Anti -Tyrosine, phosphorylated (Phospho-Tyrosine) (HRP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine
kinase activity can drive malignancy and tumor formation by generating inappropriate roliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phosphotyrosine monoclonal antibodies provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
Catalog #T9230-04C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA-peptide: 1:1000
Western Blot: 1:2000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS,
0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No11C1089
HostMouse
ConcentrationNot determined
FormSupplied as a liquid in 136mM sodium chloride, 2.6mM KCl, 12mM sodium phosphate, pH7.4 dibasic, 2mg/ml BSA 50% glycerol. Labeled with horseradish peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenTyrosine, phosphorylated, containing peptides.
SpecificityRecognizes Tyrosine, phosphorylated at (P-Tyr-100). Species Crossreactivity: All.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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