| Tyrosine hydroxylase (TH) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing. |
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| Catalog # | T9237-13 |
| Applications | Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications have not been tested. |
| Recommended Dilutions | Western Bot: 1:1000 on 10ug of PC12 lysates. |
| Immunohistochemistry: 1:100-1:1000 at 4°C, 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase. Not suitable for use on paraffin embedded, human tissue. |
| Immunofluorescence: 1:100-1:1000; Fixed with 4% PFA overnight at 4°C. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb |
| Controls | Positive: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands |
| Negative: Liver |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | Not Determined |
| Form | Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Denatured tyrosine hydroxylase from rat pheochromocytoma |
| (denatured by sodium dodecyl sulfate). |
| Specificity | Recognizes rat Tyrosine Hydroxylase at ~62kD. |
| Species Crossreactivity | Mouse, ferret, feline, human, squid, Drosophila, aplysia (marine mollusc). Expected this antibody will react with most mammalian and many non-mammalian species. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
