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You are here:Home » Antibodies » Abs to Enzymes » Anti -Tyrosine Hydroxylase (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

Anti -Tyrosine Hydroxylase (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH IF
Tyrosine hydroxylase (TH) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Catalog #T9237-13
ApplicationsSuitable for use in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsWestern Bot: 1:1000 on 10ug of PC12 lysates.
Immunohistochemistry: 1:100-1:1000 at 4°C, 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase. Not suitable for use on paraffin embedded, human tissue.
Immunofluorescence: 1:100-1:1000; Fixed with 4% PFA overnight at 4°C.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Recommended Positive Control Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands
Negative Control Liver
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot Determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenDenatured tyrosine hydroxylase from rat pheochromocytoma
(denatured by sodium dodecyl sulfate).
SpecificityRecognizes rat Tyrosine Hydroxylase at ~62kD.
Species CrossreactivityMouse, ferret, feline, human, squid, Drosophila, aplysia (marine mollusc). Expected this antibody will react with most mammalian and many non-mammalian species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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