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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B

U2 auxiliary factor (U2AF) is a highly conserved, non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site1. U2AF binds to a polypyrimidine tract at the 3' splice site. Binding of U2 snRNP with the help of U2AF and other auxiliary factors, produces the first stable complex in the mammalian spliceosome pathway
Catalog #	U0800
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1-3ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	3G299
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Purity	Purified from mouse ascites fluid.
Immunogen	An amino acid peptide derived from human U2AF65.
Specificity	Reacts with the 65kD human U2AF65 protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.