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You are here:Home » Antibodies » Abs to RNA Processing » Anti -U2 Auxiliary Factor (U2AF65)

Anti -U2 Auxiliary Factor (U2AF65)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B
U2 auxiliary factor (U2AF) is a highly conserved, non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site1. U2AF binds to a polypyrimidine tract at the 3' splice site. Binding of U2 snRNP with the help of U2AF and other auxiliary factors, produces the first stable complex in the mammalian spliceosome pathway
Catalog #U0800
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3G299
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified from mouse ascites fluid.
ImmunogenAn amino acid peptide derived from human U2AF65.
SpecificityReacts with the 65kD human U2AF65 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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