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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -UBE2C (Ubiquitin-conjugating Enzyme E2 C, Ubiquitin-protein Ligase C, Ubiquitin Carrier Protein C, UbcH10)

Anti -UBE2C (Ubiquitin-conjugating Enzyme E2 C, Ubiquitin-protein Ligase C, Ubiquitin
Carrier Protein C, UbcH10)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #U1000-86E
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-QETYSKQVTSQEP, from human UBthe C Terminal of the protein sequence according to (NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1).
SpecificityRecognizes human UBE2C.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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