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Abs to Ubiquitin Ligases
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Anti -UBE2C (Ubiquitin-conjugating Enzyme E2 C, Ubiquitin-protein Ligase C, Ubiquitin Carrier Protein C, UbcH10)
Anti -UBE2C (Ubiquitin-conjugating Enzyme E2 C, Ubiquitin-protein Ligase C, Ubiquitin Carrier Protein C, UbcH10)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| Catalog # | U1000-86E | | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes | ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:32,000 | | Western Blot: 1-3ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-QETYSKQVTSQEP, from human UBthe C Terminal of the protein sequence according to (NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1). | | Specificity | Recognizes human UBE2C. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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