You are here:Home
»
Antibodies
»
Abs to Ubiquitin Ligases
»
Anti -UBE2F (NEDD8-conjugating Enzyme UBE2F, NEDD8 Carrier Protein UBE2F, NEDD8 Protein Ligase UBE2F, NEDD8-conjugating Enzyme 2, Ubiquitin-conjugating Enzyme E2 F, NCE2)
Anti -UBE2F (NEDD8-conjugating Enzyme UBE2F, NEDD8 Carrier Protein UBE2F, NEDD8 Protein
Ligase UBE2F, NEDD8-conjugating Enzyme 2, Ubiquitin-conjugating Enzyme E2 F, NCE2)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E |
|
| Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442aa UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8. | | | Catalog # | U1000-94H | | Applications | Suitable for use in ELISA. Other applications not tested. | | Recommended Dilution | ELISA: 1:32,000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to RNKVDDYIKRYAR, from the C-terminus of the protein sequence according to NP_542409. | | Specificity | Recognizes human UBE2F. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
