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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Uncoupling Protein 1 (UCP1)

Anti -Uncoupling Protein 1 (UCP1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
The regulation of body weight depends upon the calorie intake and expenditure. It is a very complex and highly regulated process. It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals. The product of obese gene (Leptin) may influence many of these processes. White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-4) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 trans-membrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic.
Catalog #U1620-09
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000. An antibody made to this epitope has detected approx. 32kD protein in brown adipose tissues (3).
ELISA: 1:10,000-1:100,000 using 50-100ng UCP1 control peptide/well.
Optimal dilutions to be determined by the researcher.
Control PeptideU1620-05 Uncoupling Protein 1, Mouse Control Peptide
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to the C-terminus of the mouse/rat UCP1 (KLH).
SpecificityRecognizes mouse Uncoupling Protein 1 (UCP1). Species sequence homology: rat-100%, golden hamster-91%, rabbit, porcine-83%, human, canine-75%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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