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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Uncoupling Protein 2 (UCP2, SLC25A8, UCPH)

Anti -Uncoupling Protein 2 (UCP2, SLC25A8, UCPH)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The regulation of body weight depends upon the calorie intake and expenditure. It is a very complex and highly regulated process. It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals. The product of obese gene (Leptin) may influence many of these processes. White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-4) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 trans-membrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic. UCP1 is a member of the mitochondrial carrier protein family, which includes the ATP/ADP translocator, the phosphate, the 2-oxoglutarate/malate and the citrate (tricarboxylate) carriers. UCP1 plays an important role in thermoregulation in hibernating animals, neonates, and it contributes to rodent cold- and diet induced thermogenesis.
Catalog #U1621-02
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot:1-10ug/ml using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng U1621-01 UCP2 control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, 0.1% BSA.
PurityPurified by affinity chromatography.
ImmunogenA 14 amino acid peptide sequence near the C-terminus of mouse UCP2 (KLH).
SpecificityRecognizes mouse Uncoupling Protein 2 (UCP2). No significant homology with UCP1 or UCP3-5. Species Sequence Homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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