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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Uncoupling Protein 3 (UCP3)

Anti -Uncoupling Protein 3 (UCP3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein (1,2). Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long (2). UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.
Catalog #U1622-10
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1-10mg/ml using Chemiluminescence technique
Immunohistochemistry: 2-20mg/ml on formaldehyde fixed tissue
ELISA: 1:10,000-1:100,000 using 50-100ng UCP32 control peptide/well
Optimal dilutions to be determined by the researcher.
Positive ControlMuscle
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4 with 0.1% BSA.
PurityPurified by affinity chromatography.
Immunogen14 amino acid peptide sequence corresponding to the C-terminus of human UCP3. Species sequence homology: porcine, canine, 100%; mouse, rat, bovine, 92%; UCP2, 73%; UCP1, 57%.
SpecificityRecognizes human UCP3 at 30-33 kD and 24-27kD. The UCP32 peptide has no significant homology with UCP1 or UCP2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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