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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Uncoupling Protein 3 (UCP3)

Anti -Uncoupling Protein 3 (UCP3)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP FC
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCP's have tissue-specific expression; UCP3 is primarily expressed in skeletal muscle. It is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of UCP3 increase when fatty acid supplies to mitochondria exceed their oxidation capacity, and the protein enables the export of fatty acids from mitochondria. UCP's contain the three solcar protein domains typically found in MACPs (1).
Catalog #U1622-21
ApplicationsSuitable for use in Western Blot, Flow Cytometry and Immunoprecipitation. Not recommended for use in Immunofluorescence or Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000-1:10,000 detects a band at ~28kD
Immunoprecipitation: 1:10-1:100
Flow Cytometry: 1:10-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Testis, A673 lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
Clone NoEPR6427
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues in human UCP3. Cellular Localization: mitochondrion inner membrane
SpecificityRecognizes human UCP3. Species crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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