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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Uncoupling Protein 5 (UCP5, BMCP1)

Anti -Uncoupling Protein 5 (UCP5, BMCP1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The regulation of body weight depends upon the calorie intake and expenditure. It is a very complex and highly regulated process. It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals. The product of obese gene (Leptin) may influence many of these processes. White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-4) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 trans-membrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic. UCP5 may be alternatively spliced to produced at least 3 transcripts: UCP5L has a unique 22-aa unique N-terminal sequence; UCP5S is missing 3 aa (23-25 aa) and UCP5SI has 31 aa insertion between TM II-IV.
Catalog #U1625-01
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide U1625-14
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide, 0.1% BSA, 40% glycerol.
PurityPurified by immunoaffinity chromotography.
ImmunogenSynthetic peptide 16aa sequence within the N-terminus of the first extracellualr region of rat UCP5 (KLH coupled).
SpecificityRecognizes rat Uncoupling Protein 5. The sequence is conserved in all 3 isoforms in rat and mouse UCP5. Has no appreciable homology with UCP1-4. Species sequence homology: human UCP5/BMCP1-94%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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