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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -Urokinase Plasminogen Activator (uPA)

Anti -Urokinase Plasminogen Activator (uPA)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Urokinase plasminogen activator (uPA) is a serine protease. It converts the abundant proenzyme plasminogen to active plasmin and plays a key role in cancer invasion and a variety of tissue remodelling processes such as wound healing, mammary gland involution and placental development(1-3). Elevated levels of uPA are associated with poor prognosis in many types of cancer (2-5).
Catalog #U1820-14
ApplicationsSuitable for use in ELISA, Western blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: under reduced and non-reducing conditions
Immunohistochemistry (frozen sections): 1:25
Optimal dilutions to be determined by the researcher.
Culture Medium
RPMI 1640 with 10% fetal calf serum
HybridomaNS1/AG4.1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9B14
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.4, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenNative human uPA
SpecificityRecognizes the A-chain of human uPA. Binds single and two-chain uPA, uPA/PAI-1 complex and uPA/uPAR complex. A reaction isseen with the amino terminal fragment (ATF) but not with LMW uPA. It inhibits the enzymatic activity of uPA in the fibrin plate assay. No reaction with human tissue plasminogen activator (tPA) or with any other human plasma proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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