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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

The binding region of Uroplakin III has been localized on the extracellular part of the antigen. Together with the uroplakins UP Ia, UP Ib and UP II, uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47kD) and has been shown to be a specific marker of terminal urothelial differentiation (Wu et al., 1993, 1994). About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of uroplakin III. Until now, no uroplakin staining has been found in any non-urothelial carcinoma (Moll et al., 1995). Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.
Catalog #	U2735-50
Applications	Suitable for use in Western Blot and Immunohistochemistry. Not suitable for frozen sections. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: 1:10 on paraffin-embedded tissue using a heat-induced retrieval method (e.g. microwave treatment.). Incubation Time: 1 hr at RT.
	Optimal dilution determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F161
Host	Mouse
Source	Bovine
Concentration	~0.05mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Asymmetric unit membrane (AUM) preparation from bovine urinary
bladder.
Specificity	Recognizes bovine uroplakin III present in the superficial cell layer of the urothelium. Strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder and urethra. Species Crossreactivity: human, porcine and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.