Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Urotensins are neuropeptides secreted from the urophysis in the caudal neurosecretory system of the fishes. Two bioactive Urotensins, U1 and UII have been cloned from various species including mammals. UI is 41 aa peptide, which showed prolonged hypotensive effects in mammals. It is structurally similar to mammalian corticotropin-releasing factor. UII is a vasoactive 'somatostatin-like" 12-aa cyclic peptide with similarity to somatostatin-14. Human UII is composed of only 11 aa residues, while fish and frog UII possess 12 and 13 aa, respectively. The cyclic region of the UII, which is responsible for the biological activity of the peptide, has been fully conserved from fish to human. UII expression has been observed predominantly in the spinal cord.
Catalog #	U2800-01B
	Recently, an orphan human G-protein coupled receptor homologous to rat GPR14 has been identified as the receptor for UII (2). GPR14 is expressed predominantly in cardiovascular tissue. Human UII effectively stimulated total peripheral resistance in anesthetized non-human primates, a response associated with profound cardiac contractile dysfunction. GPR14 (rat 386 aa and human 389 aa, ~75% homology) contains 7 transmembrane domains. It is most closely related to somatostatin receptor4. GPR14 is mostly expressed in the heart and pancreas, while low expression was detected in the brain.
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Hybridoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9L752
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a liquid in PBS, pH7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic human urotensin II.
Specificity	Recognizes human urotensin II.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.