Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Urotensin » Anti -Urotensin II (UII)

Anti -Urotensin II (UII)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E
Urotensins are neuropeptides secreted from the urophysis in the caudal neurosecretory system of the fishes. Two bioactive Urotensins, U1 and UII have been cloned from various species including mammals. UI is 41aa peptide, which showed prolonged hypotensive effects in mammals. It is structurally similar to mammalian corticotropin-releasing factor. UII is a vasoactive 'somatostatin-like" 12-aa cyclic peptide with similarity to somatostatin-14. Human UII is composed of only 11aa residues, while fish and frog UII possess 12 and 13aa, respectively. The cyclic region of the UII, which is responsible for the biological activity of the peptide, has been fully conserved from fish to human. UII expression has been observed predominantly in the spinal cord.
Catalog #U2800-02
Recently, an orphan human G-protein coupled receptor homologous to rat GPR14 has been identified as the receptor for UII. GPR14 is expressed predominantly in cardiovascular tissue. Human UII effectively stimulated total peripheral resistance in anesthetized non-human primates, a response associated with profound cardiac contractile dysfunction. GPR14 (rat 386aa and human 389aa, ~75% homology) contains 7 transmembrane domains. It is most closely related to somatostatin receptor4. GPR14 is mostly expressed in the heart and pancreas, while low expression was detected in the brain.
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityAffinity purified over the control-peptide Sepharose
ImmunogenA 14-aa cyclic peptide from the C-terminus of rat UII (1) was synthesized, coupled to KLH (carrier protein)
SpecificityControl peptide sequence is 93% conserved in mouse, 77% in human, 66% in frog and 53% in various fishes UII. No appreciable homology is seen with other peptides. Antibody crossreactivity in various species is not established.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links