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You are here:Home » Antibodies » Abs to Secretoglobin » Anti -Uteroglobin Related Protein 1 (UGRP1, Secretoglobin Family 3A Member 2, Pneumo Secretory Protein 1, PnSP-1)

Anti -Uteroglobin Related Protein 1 (UGRP1, Secretoglobin Family 3A Member 2, Pneumo
Secretory Protein 1, PnSP-1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
UGRP1 (also secretglobin gene family 3A2/SCGB3A2) is an 18 kD, secreted nonglycosylated polypeptide that is a member of the uteroglobulin family, UGRP subfamily. Mouse UGRP1(A) circulates as a 37 kD homodimer. Its mature form is 70 amino acids in length. There are two large alternate splice forms termed B and C. UGRP1B is a 113 aa secreted homodimer that shows a 22 aa insert between aa's 84 and 85 of the short form. UGRP1C theoretically shows a 55 aa substitution for the C-terminal seven aa of the short form. Its existence is unclear. Mature mouse UGRP1A is 86% and 81% aa identical to rat and human UGRP1, respectively.
Catalog #U7001-10
HybridomaProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E,coli-derived, recombinant mouse uteroglobin-related protein-1 (rmUGRP1; aa 22-139: Accession #Q920H1).
ApplicationsWestern Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect mouse UGRP1. Using a colorimetric detection system, the detection limit for rmUGRP1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse UGRP1. The detection limit for rmUGRP1 is approximately 25 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H174
HostRat
SourceMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived rmUGRP1
SpecificitySelected for its ability to detect rmUGRP1 in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with rhUGRP1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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