| Vaccinia virus (VACV or VV) is a large, complex enveloped virus belonging to the poxvirus family of viruses. It has a linear double-stranded DNA genome, which is approximately 190kb in length and encodes for approximately 250 genes. The dimensions of the virion are roughly 360 × 270 × 250nm. Vaccinia virus is well-known for its role as a vaccine that eradicated the smallpox disease from our world, making it the first human disease to be successfully eradicated by mankind. This endeavor was carried out by the World Health Organization under the Smallpox Eradication Program. Post eradication of smallpox, scientists have been studying vaccinia virus to use as a tool for delivering genes into biological tissues (gene therapy and genetic engineering). Moreover, due to recent concerns about smallpox resurfacing as a possible agent for bioterrorism, scientists have renewed their interests in studying vaccinia virus. |
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| Catalog # | V0500-20 |
| Applications | Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Immunohistochemistry: Proteinase K digestion is recommended with formalin-fixed paaffin-embedded sections. |
| Optimal dilutions to be determined by the researcher. |
| Also Available | V0500-10: Vaccinia Virus (Smallpox, Lister strain) Pab Rb x |
| V0500-20: Vaccinia Virus (Smallpox, Lister strain) (Biotin) Pab Rb x |
| V0500-30: Vaccinia Virus (Smallpox, Lister strain) (FITC) Pab Rb x |
| V0500-40: Vaccinia Virus (Smallpox, Lister strain) (HRP) Pab Rb x |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | ~4-5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added. Labeled with Biotin. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Lister strain (mixture of virions and infected cell polyproteins, ICPs). |
| Specificity | Recognized purified virions. Crossreactive with Lister, Wyeth, New York City and MVA strains of Vaccinia. Does not crossreact with parainfluenza (1-3), RSV, adenovirus, influenza A & B or HSV-1. Does not crossreact with uninfected cells. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
