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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -VAMP 1,2 (Vesicle-Associated Membrane Protein, Synaptobrevin)

Anti -VAMP 1,2 (Vesicle-Associated Membrane Protein, Synaptobrevin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH
Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18kD integral membrane protein localized to the cytoplasmic surface of synaptic vesicle. VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxyl-terminal tail. Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system. VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons associated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells. Evidences for VAMPís involvement in vesicular transport and neurotransmission include the requirement of its yeast homologs, SNC 1 and 2, in targeting and fusion of Golgi- derived vesicles with the plasma membrane. VAMP is also the target of clostridial neurotoxins that block neurotransmitter release from presynaptic terminals. VAMP is a component of a 20S complex implicated in vesicle docking and fusion. The formation of 20S complex involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF.
Catalog #V2024-01A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot (Colorimetric): 1:4000
Immunohistochemistry: 1:50 on human neuroblastoma tissue.
Optimal dilutions to be determined by researcher.
Positive ControlsRat Brain Tissue Extract
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone No4E240
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.
PurityPurified by ammonium sulfate pecipitation.
ImmunogenImmunoprecipitated synaptic vesicle-containing fractions from human brain homogenates.
SpecificityRecognizes human VAMP on SDS-PAGE immunoblots at ~18kD. Recognizes the VAMP1 aa1-118 and the VAMP2 aa33-96-GST fusion proteins expressed in E. coli. No reactivity was seen with a ~18kD protein in mouse liver extracts. Species Crossreactivity: mouse, rat, bovine, hamster, porcine and Xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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