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You are here:Home » Antibodies » Abs to Enzymes, Oxidase » Anti -VAP-1 (Vascular Adhesion Protein 1, Amine Oxidase Copper-Containing 3, AOC3, Semicarbazide-Sensitive Amine Oxidase, SSAO)

Anti -VAP-1 (Vascular Adhesion Protein 1, Amine Oxidase Copper-Containing 3, AOC3,
Semicarbazide-Sensitive Amine Oxidase, SSAO)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Vascular adhesion protein 1 (VAP-1), also known as AOC3 (amine oxidase copper-containing 3) or SSAO (semicarbazide-sensitive amine oxidase) is a 170 kD type II transmembrane glycoprotein consisting of two disulfide-linked 763 amino acid (aa) subunits. It is expressed on endothelium, vascular smooth muscle, and adipocytes. The extracellular domain mediates lymphocyte rolling, adhesion, possibly extravasations during inflammation, and possesses monoamine oxidase activity. Adipocytes can release a soluble form via metalloproteinase cleavage. Human VAP-1 ECD shares 83% and 88% aa sequence identity with mouse and canine VAP-1 ECD, respectively.
Catalog #V2095-03
ApplicationsWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human VAP-1. The detection limit for rhVAP-1 is approximately 25 ng/lane under non-reducing and reducing conditions.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human VAP-1. The detection limit for rhVAP-1 is approximately 0.5 ng/well. In this format, this antibody shows approximately 50% cross-reactivity with rmVAP-1.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
PurityPurified by human VAP-1 affinity chromatography.
ImmunogenSf21-derived recombinant human VAP-1 extracellular domain
SpecificitySelected for its ability to recognize human VAP-1 in direct ELISAs and Western Blots.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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