Anti -Vascular Endothelial Cadherin, phosphorylated (Tyr658) (VE-Cadherin, CD144)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| VE-cadherin is an endothelial specific adhesion molecule that is essential for the maintenance of endothelial barrier function and angiogenesis. It is linked to the actin cytoskeleton through a number of adaptor proteins including alpha, beta, and gamma catenin. Cytoskeletal dynamics and phosphorylation regulate VE-cadherin mediated cell-cell adhesion. The interaction between cytosolic c-terminal Src kinase, SH2 domain and phosphorylated tyrosine 658 site of VE-Cadherin regulates density dependent cell proliferation. Phosphorylation of tyrosine 658 has been shown to lead to uncoupling of p120-catenin from the cytoplasmic tail of VE-cadherin. This phosphorylation event is sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis. | | | Catalog # | V2109-52C | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilution to be determined by researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Form | Supplied as a liquid in PBS, pH 7.3, 50% glycerol, containing 1 mg/ml BSA, and 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography | | Immunogen | Phosphorylated VE-cadherin (Tyr658) synthetic peptide corresponding to human amino acid residues around tyrosine 658. The sequence is conserved in mouse, rat, porcine, bovine, canine, chicken and chimpanzee. | | Specificity | The antibody detects a 120kD protein of VE-cadherin. Epitope | | Species Cross Reactivity | Human | | Species Sequence Homology | This sequence is 100% homologous in mouse, rat, chicken, chimpanzee, cow, dog and pig | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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