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You are here:Home » Growth Factors, Cytokines » Growth Factors-VEGF » Vascular Endothelial Growth Factor-121, Recombinant, Human (VEGF-121)

Vascular Endothelial Growth Factor-121, Recombinant, Human (VEGF-121)

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Specifications

Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating Vasolidation via nitric oxide-dependent pathways. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF.
Catalog #V2110-04R
Recombinant Human VEGF-121 is a double, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28423 Dalton. VEGF-121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Ala-Glu.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityThe biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4ng/ml.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli.
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. . 0.1ng/ug (IEU/ug).
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. No additives. Reconstitute in ddH2O, 0.1% HSA or BSA to >100ug/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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