Anti -VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E IH |
|
| The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms- like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co- operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase. | | | Catalog # | V2110-05 | | Applications | Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry (Paraffin): 1:50-1:500. Use standard deparaffinization techniques on tissue speciens. Preheat tissues using a citrate buffer, pH 6.0 and high heat epitope retrieval techniques. Note: Do not allow tissues to dry out during the staining procedure. | | Optimal dilutions to be determined by the researcher. | | Control | Pancrea, Kidney, Adenocarcinoma | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 3F401 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant human VEGF121 (NP_003376). | | Specificity | Recognizes 34-54kD isoforms of human Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF). | | Species Crossreactivity | Rabbit, mouse and rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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