| Human endocrine gland derived vascular endothelial growth factor (EG-VEGF) is selectively expressed in steroidogenic glands and promotes growth of endocrine gland endothelium. The identification of tissue-selective angiogenic factors raises the possibility that other secreted molecules in this class exist. Consistent with such an expression pattern, the human EG-VEGF gene promoter has a potential binding site for steroidogenic factor (SF)-1, a pivotal element for steroidogenic-specific transcription. In the human ovary, the expression of EG-VEGF is temporally and spatially complementary to the expression of VEGF-A, both in the follicular and in the luteal phase, suggesting complementary and coordinated roles of these molecules in ovarian angiogenesis. Also, EG-VEGF expression correlates with vascularity in the polycystic ovary syndrome, a leading cause of infertility. |
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| Catalog # | V2110-06A |
| Recombinant Human EG-VEGF is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9.6kD. |
| Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Ile-Thr-Gly. |
| Dimers and Aggregates | 1% as determined by silver-stained SDS-PAGE gel analysis. |
| Protein Content | Protein quantitation was carried out by two independent methods: |
| 1. UV spectroscopy at 280 nm. |
| 2. Analysis by RP-HPLC, using a calibrated solution of EG-VEGF as a Reference Standard. |
| Biological Activity | The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-5ng/ml. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% BSA or HSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source | Recombinant, Human (E. coli). |
| Purity | 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug) |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a lyophilized powder with no additives. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. to a concentration of >0.1mg/ml |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
