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You are here:Home » Molecular Biology » MB-Growth Factors-VEGF » Vascular Endothelial Growth Factor, Recombinant, Rat (VEGF)

Vascular Endothelial Growth Factor, Recombinant, Rat (VEGF)


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VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeablility inducing properties specific for the vascular endothelium. Multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues have been described which arise from alternative splicing of a single gene. The smaller two isoforms, VEGF 165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell-associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Catalog #V2110-07M
SourceRecombinant corresponding to 164aa from rat Vascular Endothelial Growth Factor expressed in
Molecular Weight~38.4kD
Biological ActivityED50 range=10-100ng/ml, determined by the dose dependent proliferation of human umbilical vein cells (HUVEC).
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O or buffer, 0.1% BSA., aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity~95% (SDS-PAGE). Purified by Ion Exchange chromatography.
FormSupplied as a lyophilized powder. BSA free. Reconstitute with sterile, ddH2O or buffer, 0.1% BSA. to a concentration of 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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