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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)

Anti -VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH N
Catalog #V2110-09A
ApplicationsSuitable for use in Neutralization, ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin-fixed paraffin embedded): 5-15ug/ml with a 30 minute incubation at RT or an overnight incubation at 4ºC.
Neutralization: 0.05-0.1ug/ml yields one-half maximal
inhibition [ND50] of the biological activity (10ng/ml).
ELISA (Indirect amd Sandwich): 0.5ug/ml (100ul/well). Used in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well of recombinant hVEGF.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. For long term storage, stored at -20°C. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin:
USA
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2.
Reconstitute with sterile dH2O to a concentration 0.1-1mg/ml
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified (>98%) recombinant human VEGF.
SpecificityRecognizes Vascular Endothelial Growth Factor (VEGF)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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