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Anti -VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)
Anti -VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH N |
|
| Catalog # | V2110-09A | | Applications | Suitable for use in Neutralization, ELISA, Immunohistochemistry and Western Blot. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (Formalin-fixed paraffin embedded): 5-15ug/ml with a 30 minute incubation at RT or an overnight incubation at 4ºC. | | Neutralization: 0.05-0.1ug/ml yields one-half maximal | | inhibition [ND50] of the biological activity (10ng/ml). | | ELISA (Indirect amd Sandwich): 0.5ug/ml (100ul/well). Used in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well of recombinant hVEGF. | | Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. For long term storage, stored at -20°C. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | Country of Origin: | | USA | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder from PBS, pH 7.2. | | Reconstitute with sterile dH2O to a concentration 0.1-1mg/ml | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Highly purified (>98%) recombinant human VEGF. | | Specificity | Recognizes Vascular Endothelial Growth Factor (VEGF) | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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