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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)

Anti -VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis.
Catalog #V2110-13B
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlAngiosarcoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from N-terminal of human VEGF.
Cellular LocalizationCytoplasmic, cell surface, extracellular matrix
SpecificityRecognizes human VEGF and the 165, 189 and 121 amino acid splice variants.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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