| Vascular Endothelial Growth Factor, Human (VEGF) BioAssay™ ELISA Kit contains the key components required for the quantitative measurement of natural and/or recombinant hVEGF in a sandwich ELISA format within the range of 63-2000pg/ml. Using the ELISA protocol provided, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay hVEGF ~1000 ELISA plate wells. |
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| Catalog # | V2110-15 |
| Specificity | When tested at 50ng/ml the following antigens exhibited 100% crossreactivity: Human VEGF121, Murine VEGF |
| When tested at 50ng/ml the following antigens exhibited <1% crossreactivity: |
| Human: EG-VEGF, EGF, RANTES |
| When tested at 50ng/ml the following antigens did not exhibited significant crossreactivity: |
| Human: FGF-16, GM-SCF, PDGF-AA, PDGF-AB, PDGF-BB, SCF. |
| Murine: EGF, GM-CSF, SCF. |
| Kit Components | V2110-15A1: Capture Antibody, 1x50ug |
| V2110-15A2: Detection Antibody, 1x100ug |
| V2110-15A3: Human Recombinant VEGF Standard, 1x1ug |
| V2110-15A4: Avidin Peroxidase Conjugate, 1x60ul |
| Storage and Stability | Store kit at -20°C. Stable for 6 months at -20°C. *Note: Store V2110-15A4: |
| Upon receipt, aliquot into ten 6ul vials. Store at -20°C for up to 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
