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You are here:Home » Growth Factors, Cytokines » Growth Factors-VEGF » Vascular Endothelial Growth Factor, Recombinant, Human (VEGF, MGC70609, Vascular Endothelial Growth Factor A precursor, Vascular Permeability Factor, VEGFA, VEGF-A, VPF)

Vascular Endothelial Growth Factor, Recombinant, Human (VEGF, MGC70609, Vascular
Endothelial Growth Factor A precursor, Vascular Permeability Factor, VEGFA, VEGF-A, VPF)

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Specifications

Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating Vasolidation via nitric oxide-dependent pathways. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF.
Catalog #V2110-15D
Recombinant Human VEGF produced in HEK293 cells is a double, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 40kD.
Amino Acid SequenceAPMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Biological ActivityRecombinant VEGF is fully biologically active when compared to standards. Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-6ng/ml, corresponding to a specific activity of 1x10e6 Units/mg to 1.7x10e5Units/mg.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of BDNF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight40kD
SourceHEK293 (Human Embryonic Kidney Cell Line
Purity~95% by SDS-PAGE
FormSupplied as a lyophilized powder without additives. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of 100ug/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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